Nuvia? IMAC Resin, 25 ml 7800800
Nuvia IMAC Resin is a metal chelate affinity chromatography resin that contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The inert hydrophilic surface of the base bead and the chemical structure of NTA ensure highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+, or with other transition metals such as Zn2+ or Cu2+. The optimized particle size and high porosity allows operation at increasingly higher flow rates without compromising binding capacity. Nuvia IMAC can be used at any scale from laboratory to manufacturing, providing robust, reproducible, and efficient purifications each time.
Nuvia IMAC Resin is stable in a broad range of chemicals. It is compatible with all reagents traditionally used for histidine-tagged protein purification, including reducing agents, detergents, denaturing agents, and additives.
Features and Benefits
Easy and efficient scale up from lab to manufacturing scale purifications
Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu2+, Ni2+, Co2+, or Zn2+
Optimized particle size for high flow rate and low backpressure
Superior dynamic binding capacity at high flow rates
Withstands repeated clean-in-place/recharging cycles
Robust recovery and reproducibility
Full regulatory support
Applications and Uses of Nuvia IMAC Resin
For purifying biomolecules in a variety of workflows:
Excellent single-step purity via affinity chromatography
Quick, efficient separation of biomolecules
Contaminant removal
Robust base matrix
Nuvia IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.
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