[Fermentas]PagI(BspHI)(10U/µL)-限制性內(nèi)切酶-分子生物學(xué)
平臺授權(quán)一級代理，咨詢微信：jinshanbio
PagI (BspHI) (10 U/µL)品牌：Fermentas | 貨號：ER1282| Enzyme | PagI (BspHI) |
|---|---|
| 保存溫度 | -20℃ |
| 貨期 | 2-3天 |
| Compatible Buffer | 10x Buffer O |
| Optimal Reaction Temperature | 37° C |
| Sensitive to Heat Inactivation: | Yes |
| Methylation Sensitivity | Not CpG methylation-sensitive,Not dcm methylation-sensitive,dam methylation-sensitive |
| 5′ | T↓ | C | A | T | G | A | 3′ | ||||
| 3′ | A | G | T | A | C↑ | T | 5′ |
Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism (RFLP)• SNP
Note: Low salt, high glycerol (> 5%) concentrations, pH> 8.0 or a large excess of enzyme may result in star activity. PagI cleavage is impaired by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain such as GM2163. For methylation sensitivity, refer to product specifications.
授權(quán)一級代理請認準金山科研平臺，產(chǎn)品價格和貨期請咨詢微信：jinshanbio ，詢價請加微信：jinshanbio
? 如需詢價，請加客服QQ：1749072012 、客服微信：jinshanbio，或發(fā)送郵件到1749072012@qq.com
? 平臺為生命科學(xué)研究相關(guān)領(lǐng)域提供一站式耗材試劑儀器解決方案和采購服務(wù)，數(shù)據(jù)資源基于CC協(xié)議。
? 本文地址：http://www.gamefang.cn/thread-59818.htm
特別聲明：以上內(nèi)容(如有圖片亦包括在內(nèi))來源于授權(quán)廠家或網(wǎng)絡(luò)，如有侵權(quán)，請聯(lián)系客服刪除，本平臺不提供信息校正和存儲服務(wù)。
Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.
QQ:1749072012