三、儀器操作程序1、編輯樣本1.1 將裝有反應(yīng)液的PCR反應(yīng)管放入PCR儀,記錄樣本擺放順序。1.2 點(diǎn)擊進(jìn)入軟件左方主目錄的“Workshop”界面,選擇“Edit Plate Setup”頁面。1.3點(diǎn)擊“Samples:Whole Plate loading”,根據(jù)樣本擺放的順序和樣本屬性,選擇相應(yīng)的圖標(biāo)(Unknown和Standard),在plate上相應(yīng)位置作標(biāo)識,是標(biāo)準(zhǔn)品(Standard)的,在屏幕右下方的“Standard Quantity”中輸入相應(yīng)濃度。1.4點(diǎn)擊“Select and load fluorophores”,在“1. or deselect a fluorophore”中選擇熒光標(biāo)記,在“2.Assign a color”中選擇該標(biāo)記相對應(yīng)的顏色,然后在plate上對 1.3所選擇的孔位進(jìn)行熒光標(biāo)記。1.5 完成后,在“Plate Setup Filename:”中輸入樣本文件的文件名,點(diǎn)擊“Save this plate setup”。
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