雙染蛋白MARKERPrecision Plus Protein™ Dual Color StandPrecision Plus Protein™ Dual Color Standards #161-0374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
Description技術(shù)指標
MW size range, kD | 10–250 |
Stain type | Dual Color |
Volume, µl | 500 |
Total number of applications | 50 |
Shelf life at –20°C, months | 12 |
Loading volume on mini gels, µl | for Coomassie staining, 10 |
Loading volume on mini gels, µl | for fluorescent blot detection, 5 of 1:10 dilution |
Application Type | |
Monitoring electrophoretic separation for SDS-PAGE gels | Yes |
Molecular weight sizing on SDS-PAGE gels | Yes |
Monitoring blot transfer efficiency | Yes |
Fluorescence blots | Yes |
Molecular weight sizing on | N/A |
Loading Volume on Mini Gels | |
For Coomassie staining, µl | N/A |
For stain-free gels, µl | N/A |
For silver staining, µl | N/A |
For SYPRO Ruby staining, µl | N/A |
For blot development, µl | N/A |
StrepTactin conjugate working dilutions for colorimetric blot development | N/A |
StrepTactin conjugate working dilutions for chemiluminescence blot development | N/A |
The Precision Plus Protein™ Dual Color standards are a mixture of ten recombinant proteins (10–250 kD), including eight blue-stained bands and two pink reference bands (25 and 75 kD).
雙染蛋白MARKERPrecision Plus Protein™ Dual Color Stand
Applications and Uses
Choose Precision Plus Protein Dual Color standards for SDS-PAGE:
Monitoring electrophoretic separationMolecular weight sizingMonitoring blot transfer efficiency with easy orientation via multicolor standardsFluorescent blot detection, with blue bands visualized in the red wavelength range and pink bands in the green wavelength (prepare a 1:10 dilution of the Dual Color standard and load 5 µl per well)
More Information
Loading buffer:30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue
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