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百日咳毒素Pertussis toxin P2980 名稱 品牌 規格 原始型號 價格 Sigma 百日咳毒素Pertussis toxin P2980 EAE模型 多發性硬化模型EAE Sigma 百日咳毒素Pertussis toxin P2980 Pertussis toxin from Bordetella pertussis Sigma 50UG P2980 Pertussis toxin from Bordetella pertussis Sigma 0.2MG P2980 Pertussis toxin from Bordetella pertussis buffered aqueous glycerol solution Synonym: Histamine-sensitizing factor, IAP, Islet Activating Protein, Pertussigen CAS Number: 70323-44-3 MDL number: MFCD00131841 百日咳毒素Pertussis toxinDescription Physical form Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5 Application The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al. 百日咳毒素Pertussis toxin Biochem/physiol Actions Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels. 性質 sterility aseptically packaged form buffered aqueous glycerol solution storage temp. ?20°C 百日咳毒素 Pertussis toxin from Bordetella pertussis Product Name Pertussis toxin from Bordetella pertussis, buffered aqueous glycerol solution Product Number P2980 Product Brand SIGMA CAS Number 70323-44-3 Storage Temp ?20°C TEST SPECIFICATION Purity Pass Migrates as five distinct bands when run on Polyacrylamide SDS-Urea gels. Miscellaneous Assay ≤0.33 CHO Cell Assay Lowest concentration of toxin at which a positive response （clustered growth pattern） is obtained is Concentration 0.2 mg/ml Protein by Bradford