BAPTA-AM試劑貨號：B018
O,O’-Bis(2-aminophenyl)ethyleneglycol-N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester
CAS號：126150-97-8
商品信息
儲存條件：-20度保存
運輸條件：室溫
分子式：
C34H40N2O18
分子量：
764.68
特點：
● 螯合率高于EGTA
● 可螯合細胞內的鈣離子
選擇規格：
25mg
性質
BAPTA-AM是BAPTA羧基的乙酰氧基甲基酯化產物，可以很容易地吸收到細胞中。 該酯被細胞內酯酶分解，顯示出主體在細胞中作為BAPTA的功能。 生成的BAPTA保留在細胞內部。
參考文獻
1) R. Y. Tsien, “New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures”,Biochemistry,1980,19(11), 2396.
2) R. Y. Tsien, “A Non-disruptive Technique for Loading Calcium Buffers and Indicators into Cells”,Nature,1981,290, 527.
3) J. I. Korenbrot, D. L. Ochs, J. A. Williams, D. L. Miller and J. E. Brown, “The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions”,Soc. Gen. Physiol. Ser.,1986,40, 347.
4) S. M. Harrison and D. M. Bers, “The Effect of Temperature and Ionic Strength on the Apparent Ca-affinity of EGTA and the Analogous Ca-chelators BAPTA and Dibromo-BAPTA”,Biochim. Biophys. Acta,1987,925, 133.
5) E. W. Gelfand and R. K. Cheung, “Dissociation of Unidirectional Influx of External Ca2+and Release from Internal Stores in Activated Human T Lymphocytes”,Eur. J. Immunol.,1990,20, 1237.
6) J. P. Kao, J. M. Alderton, R. Y. Tsien and R. A. Steinhardt, “Active Involvement of Ca2+in Mitotic Progression of Swiss 3T3 Fibroblasts”,J. Cell Biol.,1990,111, 183.
7) M. L. Schubert and J. Hightower, “Functionally Distinct Muscarinic Receptors on Gastric Somatostatin Cells”,Am. J. Physiol.,1990,258, G982.
8) Y. Tojyo and Y. Matsumoto, “Inhibitory Effects of Loading with the Calcium-chelator 1, 2-Bis(o-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA) on Amylase Release and Cellular ATP Level in Rat Parotid Cells”,Biochem. Pharmacol.,1990,39(11), 1775.
規格性狀
規格性狀：
該產品為無色液體。
純度（HPLC）：98.0％以上
處理條件
1.危險物第4類，第3石油類，危險等級Ⅲ 2.無火 3.儲存方法：冷凍，避光
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