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The BCS (Basic Chemical Space) Screen developed at theStructural Genomic Consortium (SGC), Oxfordhas a novel approach to screening. PEGs are grouped by molecular weight and mixed to create fourPEG Smears*(available to purchase separately). The Smears cover a broader range of chemical space while reducing the number of PEG variables.The benefit of using PEG smears is that it reduces the number of PEG variables while maintaining a large coverage of PEG space.

The bottom line is that in tests at the SGC with 191 human proteins, the BCS Screen had a 42% hit rate with only 96 conditions. Many of these proteins had failed to crystallize in other screens.

PEG Smears for broader chemical space coverage

牛津大學(xué)結(jié)構(gòu)基因組聯(lián)盟(SGC)開發(fā)的BCS(基本化學(xué)空間)篩選有一種新穎的篩選方法。PEG按分子量分組并混合以創(chuàng)建四個PEG涂片*(可單獨購買)。涂片覆蓋了更廣泛的化學(xué)空間，同時減少了PEG變量的數(shù)量。使用PEG涂片的好處是，它減少了PEG變量的數(shù)量，同時保持了較大的PEG空間覆蓋率。

底線是，在SGC對191種人類蛋白質(zhì)的測試中，BCS篩選在僅96種條件下的命中率為42%。這些蛋白質(zhì)中有許多在其他篩選中無法結(jié)晶。

MD1-104The BCS Screen96 x 10 ml

MD1-104-ECOThe BCS Eco Screen96 x 10 ml

MD1-105The BCS Screen HT-9696 x 1 mlMD1-105-ECOThe BCS Screen HT-96 Eco Screen96 x 1 mlMD1-105-FXThe BCS Screen FX pre-filled plate96 x 100 μlMD2-100-25850% v/v PEG Smear Low (100ml)100 ml

MD2-100-25950% v/v PEG Smear Medium (100ml)100 ml

MD2-100-26050% v/v PEG Smear High (100ml)100 mlMD2-100-26150% v/v PEG Smear Broad (100ml)100 ml

MD2-250-25850% v/v PEG Smear Low (250ml)250 mlMD2-250-25950% v/v PEG Smear Medium (250ml)250 ml

MD2-250-26050% v/v PEG Smear High (250ml)250 ml

MD2-250-26150% v/v PEG Smear Broad (250ml)250 mlMDSR-104-The BCS Screen Single Reagent100 mlMDSR-104-ECO-The BCS Eco Screen Single Reagent100 ml

MDSR-105-The BCS Screen HT-96 Single Reagent100 mlMDSR-105-ECO-The BCS Eco Screen HT-96 Single Reagent100 ml

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