一、 轉染小鼠胚胎成纖維細胞（NIH-3T3），轉染siRNA文章概述：
當NIH/3T3細胞生長密度達到60%，在不含FBS培養基的六孔板中進行轉染。對于細胞轉染使用5μM pGPU6/GFP/Neo-shTRIM11,siALKBH5,miR-21a-5p mimic,miR-590–5p mimic, miR-361–3p mimic, miR-202–3p mimic和相應對照，使用 Advanced DNA/RNA轉染試劑(#AD600025 Zeta life, USA) 對細胞轉染；在轉染24小時后分別對TRIM11或ALKBH5通過蛋白質印跡或qPCR檢測敲低效率。
二、其它
1、NIH/3T3細胞培養條件：文章選擇Z7181FBS-500胎牛血清培養(# Zeta life, USA)
2、文章作者：河北醫科大學公共衛生學院毒理學教研室、河北醫科大學環境與健康重點實驗室
3、標題：FcγRIIB The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM2.5 exposure-induced pulmonary fibrosis of mice .(IF10.588)
三、轉染轉染NIH-3T3細胞原文
Cell transfection
Transfection was conducted when NIH/3T3 cells grown to 60% density in six-well plates with FBS absent medium. For cell transfection, 5 μM of pGPU6/GFP/Neo-shTRIM11, siALKBH5, miR-21a-5p mimic, miR-590–5p mimic, miR-361–3p mimic, miR-202–3p mimic and cor-responding control (Genepharma, Shanghai, China) was transfected into cells using Advanced DNA/RNA Transfection Reagent (Zeta life, USA) according to the supplier’s guidelines, respectively. TRIM11 or ALKBH5 knockdown efficiency was detected by western blotting or qPCR after 24 h post-transfection, respectively.
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