品牌: wshtbio
公司: 上海萬生昊天生物技術有限公司
貨號： EZIP02-5 供應商： 上海萬生昊天生物技術有限公司 規格： 5ml
產品詳情請聯系金山科研平臺微信：jinshanbio BackgroundThe HA tag is derived from the human influenza hemagglutinin corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. This tag facilitates the detection, isolation, and purification of the proteins.Isotype Mouse IgG1FormSlurrySource Purified antibody was conjugated to gel on optimal condition.Concentration 50% (v/v) in PBS pH7.4, 0.02% Sodium Azide, 55% glycerol.Storage -20℃Applications IP, protein purificationSpecificity All speciesProcedureIsolation of HA fusion proteins with Anti-HA tag antibody Affinity Gel.1. Thoroughly suspend the Anti-HA Affinity Gel in the vial, in order to make a uniform suspension of the resin. Quickly transfer 10μl of the gel suspension (about 5μl of packed gel volume) to a fresh tube.2. Add 0.6ml TBS. Thoroughly suspend the Anti-HA Affinity Gel. Centrifuge the resin at 10000 rpm for 30 seconds. Remove the supernatant carefully. Be sure that most of the wash buffer is removed and no resin is discarded. This step should be repeated for 3-4 times.3. Add 500μl of E .coli periplasmic extracts to the washed resin.4. Agitate all samples gently for 2 hours at 4℃.5. Centrifuge the resin for 30 seconds at 10000 rpm. Remove the supernatants to a fresh tube.6. Wash the resin with 0.5ml TBS until the OD280 of the supernatant liquid＜0.05.7. Add 10μl of 2×sample buffer to each sample (the resin and the supernatant). Boil the samples for 5 minutes.8. Load 4μl of each sample on SDS-PAGE.FOR RESEARCH USE ONLY.Related Products
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